Difference between revisions of "Part:BBa K2996501"
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<center><b>Figure 1.</b> <i>PCR product of DBD with Flag tag(421bp). The ladder represents the size of DNA in bp.</i> </center>
 
<center><b>Figure 1.</b> <i>PCR product of DBD with Flag tag(421bp). The ladder represents the size of DNA in bp.</i> </center>

Revision as of 15:06, 21 October 2019

_NOTOC__ Terminator put downstream of zinc finger

It is composite part composed of BBa_K2996500 and BBa_B0010.This is used as a half of transcription unit.We aimed to combine part BBa_K2996502 and this part to make an entire production module of C-luciferase-DBD fusion protein. We used PCR to construct this part.As for the terminator,we used primers to bring it to part BBa_K2996500.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Figure 1. PCR product of DBD with Flag tag(421bp). The ladder represents the size of DNA in bp.