Difference between revisions of "Part:BBa K3286104"

Latest revision as of 14:54, 21 October 2019

Expression cassette for small proteins in E. coli BL21DE3

Use this part, containing a T7 promoter (BBa_K1614000), Bicistronic Design (BCD) system [1](BBa_M36516), Strep tag, MBP (Maltose Binding Protein) and TEV (Tomato Etch Virus) site (BBa_K3286103) to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.

[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 493
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 191

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3286104 short</partinfo>
-Use this part, containing a T7 promoter, BCD system, Strep tag, MBP and TEV site to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.
+Use this part, containing a T7 promoter (<partinfo>BBa_K1614000</partinfo>), Bicistronic Design (BCD) system [1](<partinfo>BBa_M36516</partinfo>), Strep tag, MBP (Maltose Binding Protein) and TEV (Tomato Etch Virus) site (<partinfo>BBa_K3286103</partinfo>) to produce small protein domains in high volumes. After isolation of the fusion protein and treatment with TEV protease, run it again over a strep column to remove the Maltose binding protein.
+[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.
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