Difference between revisions of "Part:BBa K2996503"
Line 1: Line 1:
  
−
_NOTOC__
+
__NOTOC__
 
<partinfo>BBa_K2996503 short</partinfo>
 
<partinfo>BBa_K2996503 short</partinfo>
  

Revision as of 14:53, 21 October 2019


The part is aimed to create a fused protein of C-luciferase and zincfinger

This part is made up of part BBa_K2996502 and part BBa_K2996501,which produces a fused protein of C-luciferase and zincfinger.We used overlapping PCR to produce the DNA fragment.The fused protein is aimed to bring the C-luciferase to the site of target sequence of zincfinger.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 846
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 302
    Illegal AgeI site found at 441
  • 1000
    COMPATIBLE WITH RFC[1000]


< img style="max-width: 50%" src="https://2019.igem.org/wiki/images/8/8d/T--SJTU-BioX-Shanghai--jsf-CDBD.png" alt="" />
Figure 1. overlap PCR product of C-luciferase-DBD(963bp). The ladder represents the size of DNA in bp.