Difference between revisions of "Part:BBa K2971001:Design"

 
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===Design Notes===
 
===Design Notes===
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Make sure not to have restriction sites
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The gene was selected from Deinococcus radiodurans due to the organism’s extremophile properties. D.radiodurans exhibit several traits that would make it ideal in our imagined biogenic solar cell design (see our design page), such as high resistance towards ionizing radiation and dehydration.  Although, the translated protein does not necessarily exhibit the same resistances as the whole organism, it was selected to highlight our interest in D.radiodurans.
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The gene was placed in an expression vector under pBAD induction so that we could over-express and purify the protein to test the enzymatic activity of the protein. Due to time restraints, and the general difficulty of characterizing carotenogenic enzymes, this was not achieved.
  
  
 
===Source===
 
===Source===
  
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Gene synthesized by company. Sequence taken from Deniococcus radiodurans.
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Gene synthesized by Integrated DNA Technologies. Sequence taken from Deniococcus radiodurans.
  
 
===References===
 
===References===

Latest revision as of 14:36, 21 October 2019


crtI from Deinococcus radiodurans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 955
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was selected from Deinococcus radiodurans due to the organism’s extremophile properties. D.radiodurans exhibit several traits that would make it ideal in our imagined biogenic solar cell design (see our design page), such as high resistance towards ionizing radiation and dehydration. Although, the translated protein does not necessarily exhibit the same resistances as the whole organism, it was selected to highlight our interest in D.radiodurans. The gene was placed in an expression vector under pBAD induction so that we could over-express and purify the protein to test the enzymatic activity of the protein. Due to time restraints, and the general difficulty of characterizing carotenogenic enzymes, this was not achieved.


Source

Gene synthesized by Integrated DNA Technologies. Sequence taken from Deniococcus radiodurans.

References