Difference between revisions of "Part:BBa K3286106"
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[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016.  
 
[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016.  
  
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Chitin binding assay
 
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<html>
 
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<figure>
 
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<img style="width:50%;" src="https://2019.igem.org/wiki/images/2/23/T--Wageningen_UR--Partspage_BBa_K3286106.jpeg">
 
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<figcaption>
 
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<b> Figure 1:</b> Protein concentration in supernatant in ug/ml. 55% of PD1764sh has bound to chitin in this assay. The influence of chitin on protein concentration in general is negligible, as the BSA sample indicates
 
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</figcaption>
 
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</figure>
 
  
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3286106 parameters</partinfo>
 
<partinfo>BBa_K3286106 parameters</partinfo>
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Chitin binding assay
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<html>
 +
<figure>
 +
<img style="width:50%;" src="https://2019.igem.org/wiki/images/2/23/T--Wageningen_UR--Partspage_BBa_K3286106.jpeg">
 +
<figcaption>
 +
<b> Figure 1:</b> Protein concentration in supernatant in ug/ml. 55% of PD1764sh has bound to chitin in this assay. The influence of chitin on protein concentration in general is negligible, as the BSA sample indicates
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</figcaption>
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</figure>
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Revision as of 14:29, 21 October 2019


Shorter version of PD1764

This protein is a shorter (sh) version of BBa_K3286105. Due to isolation troubles, the first 20 amino acids have been removed from this protein. The part contains a chitin-binding domain that is essential for chitin binding capacities [1].

[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 787
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]