Difference between revisions of "Part:BBa K2971001"

 
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<partinfo>BBa_K2971001 short</partinfo>
 
<partinfo>BBa_K2971001 short</partinfo>
  
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The part is a phytoene desaturase
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This part is the gene crtE (dr1395) from the extremophile Deinococcus radiodurans. The gene encodes geranylgeranyl diphosphate synthase (GGPPS). GGPPS catalyze the condensation of farnesyl pyrophosphate (FPP) and isopentenyl pyrophosphate (IPP) into geranylgeranyl diphosphate (GGPP). GGPP is a precursor for the carotenoid biosynthetic pathway. The sequence has been codon optimized for expression in Escherichia coli and iGEM registry compatibility.
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The UiOslo 2019 team used this gene in a composite part to produce the red pigment lycopene, in Escherichia coli, by expressing it in a vector along with the genes crtB and crtI.
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Revision as of 14:28, 21 October 2019


crtI from Deinococcus radiodurans

This part is the gene crtE (dr1395) from the extremophile Deinococcus radiodurans. The gene encodes geranylgeranyl diphosphate synthase (GGPPS). GGPPS catalyze the condensation of farnesyl pyrophosphate (FPP) and isopentenyl pyrophosphate (IPP) into geranylgeranyl diphosphate (GGPP). GGPP is a precursor for the carotenoid biosynthetic pathway. The sequence has been codon optimized for expression in Escherichia coli and iGEM registry compatibility.

The UiOslo 2019 team used this gene in a composite part to produce the red pigment lycopene, in Escherichia coli, by expressing it in a vector along with the genes crtB and crtI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 955
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]