Difference between revisions of "Part:BBa K2999000"

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<partinfo>BBa_K2999000 short</partinfo>
 
<partinfo>BBa_K2999000 short</partinfo>
  
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This part is mainly used to encode the extracellular polysaccharide hydrolase PelA of Pseudomonas aeruginosa PAO1 biofilm and its hydrolysis. The first 100 bp of the sequence is its own RBS region.
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This part is mainly used to encode the extracellular polysaccharide hydrolase PelA and its hydrolysis of biofilm produced by ''Pseudomonas aeruginosa'' PAO1 . The first 100 bp of the sequence is its own RBS region.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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Please click:<partinfo>BBa_K2999003</partinfo>
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Please click:<html><a href='https://parts.igem.org/Part:BBa_K2999009'>BBa_K2999009</a></html>
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YAU-China expressed the biofilm-damaged system in 2019, using extracts from each part of the system for experiments.
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After overnight induction of the control strain, two strains expressing single enzyme and two strains co-expressing two enzymes, four strains were collected by centrifugation and suspended in PBS buffer of equal volume. The supernatant was absorbed and added into the biofilm of Pseudomonas aeruginosa cultured on the pre-PVC plate. After incubation for a period of time, the PVC plate was cleaned, stained with crystal violet for 10 minutes, and the remaining biomass was treated with anhydrous ethanol. The coatings were washed out and OD570 was detected by enzyme labeling.
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[[File:T--YAU-China--iGEM2019-BBa -4.jpg|500px|thumb|left|Figure 1. Effects of PelA and PslG on Biofilm]]
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[[File:T--YAU-China--iGEM2019-BBa -3.png|500px|thumb|left|Figure 2. Crystal violet staining of biofilm treated with extracts from control group and three groups]]
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Latest revision as of 14:27, 21 October 2019


RBS+PelA

This part is mainly used to encode the extracellular polysaccharide hydrolase PelA and its hydrolysis of biofilm produced by Pseudomonas aeruginosa PAO1 . The first 100 bp of the sequence is its own RBS region.


Usage and Biology

Please click:BBa_K2999009

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2427
    Illegal BamHI site found at 1410
    Illegal BamHI site found at 1575
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 191
    Illegal NgoMIV site found at 215
    Illegal NgoMIV site found at 236
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 563
    Illegal NgoMIV site found at 1271
    Illegal NgoMIV site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1157
    Illegal BsaI site found at 1783
    Illegal BsaI.rc site found at 1358