Difference between revisions of "Part:BBa K3093003:Design"
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===Design Notes=== | ===Design Notes=== | ||
The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site and change the old -35 signal "TACTAT" to the new -35 signal "TTGACA" | The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site and change the old -35 signal "TACTAT" to the new -35 signal "TTGACA" | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | [1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032. | ||
Latest revision as of 14:16, 21 October 2019
chbB promoter with a new -35
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site and change the old -35 signal "TACTAT" to the new -35 signal "TTGACA"
Source
Escherichia coli str. K-12 substr.MG1655.
References
[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.