Difference between revisions of "Part:BBa K3117006"

Revision as of 14:00, 21 October 2019

Igk leader sequence

The Igk leader is a signaling sequence, directing the protein into the secretory pathway.

Sequence and Features

Assembly Compatibility:

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 <partinfo>BBa_K3117006 short</partinfo>
-The part BBa_K3117006 is a secretory leader sequence for eucaryotic expression. With the help of Igk, proteins can be directly secreted and harvested out of the supernatant. Igk is normally fused to the N-terminus of the protein of interest.
+The Igk leader is a signaling sequence, directing the protein into the secretory pathway.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies).
+The Igk leader is a signaling sequence, directing the protein into the secretory pathway (Feng, Gong, & Dimitrov, 2011). By adding this signaling peptide the protein of interest can be secreted in the supernatant after transfection and the basis for a proper protein folding can provided. Prior to secretion the Igk leader is cleaved of. Igk leader is normally fused to the N-terminus of the protein of interest.
+The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies)after HEK293T cell transfection.
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 <span class='h3bb'>Sequence and Features</span>