Difference between revisions of "Part:BBa K091182"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K091182 short</partinfo> | <partinfo>BBa_K091182 short</partinfo> | ||
| − | This | + | This construct uses two hybrid promoters. pLas/cI drives expression of the LuxI and Mnt genes. The promoter is designed to respond to activation by LasR when bound to P-AI-1 and to repression by lambda cI. pTet/Mnt drives expression of the LuxI and cI genes. It is designed for repression by the Tet repressor, which can be derepressed by aTc, and by the Mnt repressor. LuxI is a sythase that produces AI-1. |
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 16:43, 25 October 2008
pLasR/cI-RBS-LuxI-RBS-Mnt-TT-pMnt/tetR-RBS-LuxI-RBS-cI-TT
This construct uses two hybrid promoters. pLas/cI drives expression of the LuxI and Mnt genes. The promoter is designed to respond to activation by LasR when bound to P-AI-1 and to repression by lambda cI. pTet/Mnt drives expression of the LuxI and cI genes. It is designed for repression by the Tet repressor, which can be derepressed by aTc, and by the Mnt repressor. LuxI is a sythase that produces AI-1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 58
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 827
Illegal BglII site found at 1356
Illegal BglII site found at 2078
Illegal XhoI site found at 1318 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]