Difference between revisions of "Part:BBa K2905016"

Latest revision as of 13:41, 21 October 2019

High pH Sensor

This part is to be used as a reporter gene that is active at high pH levels.

Measuring Expression/Fluorescence at different pH: Alma 2019

To determine if the part works as intended (expression at high pH, repression at pH less than 5.5), K2905016 (this part) or a control, BBa_J04450 were analyzed at different pH. Cells were grown to mid-log in LB, then pelleted and transferred to M9 minimal media with pH adjusted to ~4.5 or left untouched at 7, and then grown for about 14 hours shaking at 37C. Fluorescence was measured in a Mx3500.

As can be seen from the graph, there is a statistically significant difference between expression for the reporter when comparing acidic and neutral conditions. From this we would conclude that the reporter is working as intended, at least qualitatively - compare this data to the inverse part, BBa_K2762014.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1661
Illegal AgeI site found at 1773
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 332

@@ Line 5: / Line 5: @@
 This part is to be used as a reporter gene that is active at high pH levels.
-<!-- Add more about the biology of this part here
+===Measuring Expression/Fluorescence at different pH: Alma 2019===
-===Usage and Biology===
+To determine if the part works as intended (expression at high pH, repression at pH less than 5.5), K2905016 (this part) or a control, [https://parts.igem.org/Part:BBa_J04450 BBa_J04450] were analyzed at different pH. Cells were grown to mid-log in LB, then pelleted and transferred to M9 minimal media with pH adjusted to ~4.5 or left untouched at 7, and then grown for about 14 hours shaking at 37C. Fluorescence was measured in a Mx3500.
+[[File:T--Alma--high_pH_graph1.png|800px]]
+As can be seen from the graph, there is a statistically significant difference between expression for the reporter when comparing acidic and neutral conditions. From this we would conclude that the reporter is working as intended, at least qualitatively - compare this data to the inverse part, [https://parts.igem.org/Part:BBa_K2762014 BBa_K2762014].
 <!-- -->