Difference between revisions of "Part:BBa K3002126"

Revision as of 13:33, 21 October 2019

Level 1 � Wildtype-PETase + His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the His-tag (BBa_K3002028)and the coding sequence of the Wildtype-PETase (BBa_K3002004) plus the MoClo connectors for positions B2-B3 (BBa_K3002003), B4-B5 (BBa_K3002004)and B5-B6 (BBa_K3002005).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1002
Illegal PstI site found at 1975
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 249
Illegal PstI site found at 1002
Illegal PstI site found at 1975
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1002
Illegal PstI site found at 1975
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1002
Illegal PstI site found at 1975
Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 764
Illegal NgoMIV site found at 2334
1000
COMPATIBLE WITH RFC[1000]

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-This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), the His-tag a href="https://parts.igem.org/Part:BBa_K3002028">BBa_K3002028</a>)and the coding sequence of the Wildtype-PETase (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>) plus the MoClo connectors for positions B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002003">BBa_K3002003</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>)and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002005">BBa_K3002005</a>).
+This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), the His-tag (<a href="https://parts.igem.org/Part:BBa_K3002028">BBa_K3002028</a>)and the coding sequence of the Wildtype-PETase (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>) plus the MoClo connectors for positions B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002003">BBa_K3002003</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>)and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002005">BBa_K3002005</a>).
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