Difference between revisions of "Part:BBa K3320005"

 
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<partinfo>BBa_K3320005 short</partinfo>
 
<partinfo>BBa_K3320005 short</partinfo>
  
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This composite parts are the basic constitution of our AND gate, repressing pTet with TetR protein regulated by phiF promoter.
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This composite part is the basic constitution of our AND gate, repressing pTet with TetR protein regulated by phiF promoter.
  
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<!-- Add more about the biology of this part here
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<!-- Add more about the biology of this part here <!-- -->
 
===Usage and Biology===
 
===Usage and Biology===
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pPhlF is an orthogonal promoter repressed by PhlF Repressor. In the absence of PhlF repressor, produced by the lactate promoter, this part is constitutively expressing TetR,  which represses the production of BBa_K3320013.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3320005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3320005 SequenceAndFeatures</partinfo>

Revision as of 13:20, 21 October 2019


Basic Repressor Unit

This composite part is the basic constitution of our AND gate, repressing pTet with TetR protein regulated by phiF promoter.

Usage and Biology

pPhlF is an orthogonal promoter repressed by PhlF Repressor. In the absence of PhlF repressor, produced by the lactate promoter, this part is constitutively expressing TetR, which represses the production of BBa_K3320013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1596