Difference between revisions of "Part:BBa K2973016:Experience"
 
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===Applications of BBa_K2973016===
 
===Applications of BBa_K2973016===
  
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In our project, this primer (BBa_K2973016) was used along with its reverse primer (BBa_K2973017) for the amplification reactions of the IS6110 gene ([[Part:BBa_K2973009]]) that was used as a biomarker, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes. These primers include 5' overhangs ( a T7 promoter and a trigger sequence) to allow for transcription by a T7 RNA Polymerase and translation by a toehold-switch regulated system encoding for a reporter gene (BBa_K2973007). The expected amplified product length is 136 bp.
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In our project, this primer (<partinfo>BBa_K2973016</partinfo>) was used along with its reverse primer (<partinfo>BBa_K2973017</partinfo>) for the amplification reactions of the IS6110 gene (<partinfo>BBa_K2973009</partinfo>) that was used as a biomarker, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes. These primers include 5' overhangs ( a T7 promoter and a trigger sequence) to allow for transcription by a T7 RNA Polymerase and translation by a toehold-switch regulated system encoding for a reporter gene (<partinfo>BBa_K2973007</partinfo>). The expected amplified product length is 136 bp.
  
  

Latest revision as of 13:01, 21 October 2019


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Please enter how you used this part and how it worked out.

Applications of BBa_K2973016

In our project, this primer (BBa_K2973016) was used along with its reverse primer (BBa_K2973017) for the amplification reactions of the IS6110 gene (BBa_K2973009) that was used as a biomarker, in order to perform isothermal amplification reactions with Recombinase Polymerase Amplification (RPA). Using this method, we were able to detect quantities as low as 10 to the minus 9 nanograms in reaction times ranging from five to 20 minutes. These primers include 5' overhangs ( a T7 promoter and a trigger sequence) to allow for transcription by a T7 RNA Polymerase and translation by a toehold-switch regulated system encoding for a reporter gene (BBa_K2973007). The expected amplified product length is 136 bp.


RPA reaction after clean up of the amplified product:

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PCR reaction:

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User Reviews

UNIQ606643a462165141-partinfo-00000006-QINU UNIQ606643a462165141-partinfo-00000007-QINU