Difference between revisions of "Part:BBa K2989007"
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Ce-HBsAg-125 is located in the downstream region of the hulc promoter. After specific initiation in tumor cells, the downstream Ce-HBsAg-125 is expressed. Ce-HBsAg-125 can competingly inhibit miR-HBsAg-125 to allow transcription of HBsAg It, together with miR-HBsAg-125, helps to form a regulation system. | Ce-HBsAg-125 is located in the downstream region of the hulc promoter. After specific initiation in tumor cells, the downstream Ce-HBsAg-125 is expressed. Ce-HBsAg-125 can competingly inhibit miR-HBsAg-125 to allow transcription of HBsAg It, together with miR-HBsAg-125, helps to form a regulation system. | ||
| + | https://2019.igem.org/wiki/images/e/ef/T--Nanjing_NFLS--Parts_BBa_K2989007.jpg | ||
| + | A: pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125 <br/> | ||
| + | B: pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125 co-transformed with pCDNA3.1(+)-Hulc-CeR-125 <br/> | ||
| + | We transfected plasmids into HepG2 cells: in Figure A, we transfected pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125 alone while in Figure B, we co-transfected pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125 and pCDNA3.1(+)-Hulc-CeR-125 plasmid. The figures showed that the EmGFP brightness significantly increased after we co-transfected the Ce-HBsAg-125 part, demonstrating that Ce-HBsAg-125 worked properly. | ||
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Latest revision as of 13:00, 21 October 2019
Ce-HBsAg-125
Competitive endogenous RNA (ceRNA) hypothesis describes a complex post-transcriptional regulatory network that includes lncRNA, mRNA, and other types of RNA. They act as natural miRNA sponges that inhibit the function of other RNA by competing for one or more miRNA binding sites.
Usage
Ce-HBsAg-125 is located in the downstream region of the hulc promoter. After specific initiation in tumor cells, the downstream Ce-HBsAg-125 is expressed. Ce-HBsAg-125 can competingly inhibit miR-HBsAg-125 to allow transcription of HBsAg It, together with miR-HBsAg-125, helps to form a regulation system.
A: pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125
B: pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125 co-transformed with pCDNA3.1(+)-Hulc-CeR-125
We transfected plasmids into HepG2 cells: in Figure A, we transfected pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125 alone while in Figure B, we co-transfected pCDNA6.2-hTERT-HBsAg-EmGFP-miR-125 and pCDNA3.1(+)-Hulc-CeR-125 plasmid. The figures showed that the EmGFP brightness significantly increased after we co-transfected the Ce-HBsAg-125 part, demonstrating that Ce-HBsAg-125 worked properly.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]