Difference between revisions of "Part:BBa K2973015"

Latest revision as of 12:58, 21 October 2019

Hepatitis B Virus fragment (281-560bp)

Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic diseases. This part represents a small fragment of the virus's genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.

Usage and Biology

This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfully amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).

PCR Reaction:

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RPA reaction after cleanup of the amplified product:

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 210
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2973015 short</partinfo>
-Hepatitis B is a DNA virus that attacks the liver and can cause both acute and chronic disease.
+<i>Hepatitis B</i> is a DNA virus that attacks the liver and can cause both acute and chronic diseases.
-This part represents a small fragment of the virus' genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.
+This part represents a small fragment of the virus's genome. It is a small DNA fragment(280 bp), that does not encode for any functional protein or toxin. It's a small part of a sequence that encodes for a DNA polymerase of the virus and in alternative reading frames it encodes for envelope proteins of the virus, or for a specific surface antigen.
 ===Usage and Biology===
-This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfuly amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).
+This part serves as a DNA template for amplification reactions (biomarker). In our project, we successfully amplified a specific region of the part using PCR and Recombinase Polymerase Amplification (RPA).
 PCR Reaction:
@@ Line 35: / Line 35: @@
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 <span class='h3bb'>Sequence and Features</span>