Difference between revisions of "Part:BBa K2974400:Design"
Line 11: Line 11:
 
[[File:T--Lambert_GA--Lin4Megan.png|800px]]
 
[[File:T--Lambert_GA--Lin4Megan.png|800px]]
 
</center>
 
</center>
 +
<br>
 
<center><i>Figure 1: BLAST results for C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)</i></center>
 
<center><i>Figure 1: BLAST results for C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)</i></center>
 
<br>
 
<br>
Line 19: Line 20:
 
[[File:T--Lambert GA--ToeholdMegan1.png|800px]]
 
[[File:T--Lambert GA--ToeholdMegan1.png|800px]]
 
</center>
 
</center>
 +
<br>
 
<center><i>Figure 2: Complementary trigger RNA sequence derived from C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)</i></center>
 
<center><i>Figure 2: Complementary trigger RNA sequence derived from C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)</i></center>
  

Revision as of 12:48, 21 October 2019


T7 C. elegans Trigger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 159
    Illegal NotI site found at 6
    Illegal NotI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69


Design Notes

T--Lambert GA--Lin4Megan.png


Figure 1: BLAST results for C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)




T--Lambert GA--ToeholdMegan1.png


Figure 2: Complementary trigger RNA sequence derived from C. elegans lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic)


Source

The lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic) is native to the genome of C. elegans.

References