Difference between revisions of "Part:BBa K104001:Design"

 
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===References===
 
===References===
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C Klein, C Kaletta, N Schnell and K D Entian (1992).Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol. 58(1): 132-142

Revision as of 10:04, 25 October 2008

Sensor for small peptide Subtilin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A terminator was included after the spaRK to prevent read through. The native SpaRK promoter is controlled by the sigma factor sigma-H and induced at the end of exponential phase. The regulation by sigma-H was left unmodified.


Source

The two component system, its promoter and RBS's, and the pSpaS promoter sequence are all derived from the genome of Bacillus subtilis ATCC 6633.

References

C Klein, C Kaletta, N Schnell and K D Entian (1992).Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol. 58(1): 132-142