Difference between revisions of "Part:BBa K3110046"
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\FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to RPMrel. We amplified the FimHRPMrel from this existing part, and this can be used in combination with different tags and/or promoter/RBS sequences. | \FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to RPMrel. We amplified the FimHRPMrel from this existing part, and this can be used in combination with different tags and/or promoter/RBS sequences. | ||
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Latest revision as of 12:27, 21 October 2019
FimH 136KO-RPMrel
\FimH protein is a subunit of type 1 pili present in E coli. The FimH adhesin found at the tip of type 1 pili binds naturally to mannose. Substitution of 1 amino acid at the 136th position disrupts its ability to bind to mannose. Introduction of the above mutation and adding RPMrel, a colon cancer homing peptide downstream ensures it selective binding to colon cancer cells. This part was already developed with an arabinose promoter, a SpyTag and a HisTag downstream to RPMrel. We amplified the FimHRPMrel from this existing part, and this can be used in combination with different tags and/or promoter/RBS sequences.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 496
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]