Difference between revisions of "Part:BBa K3102011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Sequence synthesized and optimized for E.coli on | + | Sequence synthesized and optimized for E.coli on Twist Bioscience |
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===Source=== | ===Source=== | ||
Latest revision as of 12:13, 21 October 2019
EG1 (Endo-1,4-beta-glucanase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1112
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 55
Illegal AgeI site found at 313
Illegal AgeI site found at 403
Illegal AgeI site found at 1240 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence synthesized and optimized for E.coli on Twist Bioscience
Source
Neisseria Sicca, sequence found through genebank https://www.ncbi.nlm.nih.gov/protein/BAJ21449.1
References
Moriyoshi K, Ohmoto T, Ohe T, Sakai K. "Role of Endo-1,4-β-glucanases from Neisseria sicca SB in synergistic degradation of cellulose acetate". Biosci Biotechnol Biochem. 2003;67(2):250–7.