Difference between revisions of "Part:BBa K108001:Design"

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===Design Notes===
 
===Design Notes===
cloned into pSB1AC3
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 +
PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
  
 
===Source===
 
===Source===
  
Enterobacteria phage lambda
+
S, R and Rz were cloned from Enterobacteria phage lambda genome (reference 1 and 2).
  
 
===References===
 
===References===
  
1. NCBI
+
1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311.
 +
 
 +
2. NCBI

Latest revision as of 07:32, 25 October 2008

SRRz


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.

Source

S, R and Rz were cloned from Enterobacteria phage lambda genome (reference 1 and 2).

References

1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311.

2. NCBI