Difference between revisions of "Part:BBa K3030003"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3030003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3030003 SequenceAndFeatures</partinfo>
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EAAT2-Functional Characterization
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At the beginning of our project, we characterized the function of EAAT2 and its expression on pcDNA3.1(+), and simulated the inflammation-inducing environment for Neuro-2a cells to mimic the high-glutamate condition in neurodegenerative patients’ cerebrospinal fluid.
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In this part of test, we focused on two impacts our exosome medicine may bring to the patients:
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Reducing the excitatory amino acids in cerebrospinal fluid.
 +
Alleviate the cytotoxicity brought by over-high glutamate or the excitotoxicity induced.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 11:29, 21 October 2019


Excitatory Amino Acid Transporter 2 with C/D Box on pcDNA 3.1+

This part contains EAAT2 protein that may selectively transport glutamate, which is an universal excitatory neurotransmitter, from cleft and extracellular matrix. The high expression of EAAT2 can significantly alleviate the excitotoxicity induced by the overhigh glutamate. The part also contains C/D box which may binds to L7Ae transfected and expressed on inner side of exosome membrane, and help hold the mRNA of EAAT2 on exosomes.The vector of this part is pcDNA3.1+ that can be expressed in eukaryotes, which includes the promoter CMV.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal PstI site found at 5826
    Illegal PstI site found at 7120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal PstI site found at 5826
    Illegal PstI site found at 7120
    Illegal NotI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BamHI site found at 929
    Illegal XhoI site found at 985
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal PstI site found at 5826
    Illegal PstI site found at 7120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 991
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2313
    Illegal PstI site found at 5826
    Illegal PstI site found at 7120
    Illegal NgoMIV site found at 1423
    Illegal NgoMIV site found at 2764
    Illegal NgoMIV site found at 3047
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4572
    Illegal SapI site found at 3492
    Illegal SapI.rc site found at 2613
    Illegal SapI.rc site found at 2823

EAAT2-Functional Characterization At the beginning of our project, we characterized the function of EAAT2 and its expression on pcDNA3.1(+), and simulated the inflammation-inducing environment for Neuro-2a cells to mimic the high-glutamate condition in neurodegenerative patients’ cerebrospinal fluid. In this part of test, we focused on two impacts our exosome medicine may bring to the patients: Reducing the excitatory amino acids in cerebrospinal fluid. Alleviate the cytotoxicity brought by over-high glutamate or the excitotoxicity induced.