Difference between revisions of "Part:BBa K2974400:Experience"
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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<strong>T7 C. elegans Trigger</Strong>
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<center><img src="https://2019.igem.org/wiki/images/9/96/T--Lambert_GA--FluoroData4.png" class="midPic pic">
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<p class="bodytext caption midPic"> This graph compares the mean lux values of the </i>C. elegans<i> toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence. </i></center>
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The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the trigger. In addition, sequencing analysis results shown below also confirms the presence of the trigger in comparison to the trigger sequences in the reference paper (Green et al. 2014). The biobricked trigger's (BBa_K2974400) function has been validated through experimentation and sequencing.
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<center><img src="https://2019.igem.org/wiki/images/9/96/T--Lambert_GA--FluoroData4.png" height="300px"></center>
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<center><I>Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.</I></center>
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<strong>Sequencing Analysis</strong>
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<center><img src="https://2019.igem.org/wiki/images/1/1a/T--Lambert_GA--CETriggerSequenceMegan.png" height="400px">
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<i><center>Figure 2: Lambert iGEM aligned the Trigger insert with the Eurofins Genomics sequencing results. This showed that the Trigger was successfully cloned.</center></i>
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===Applications of BBa_K2974400===
 
===Applications of BBa_K2974400===
  

Latest revision as of 11:13, 21 October 2019


T7 C. elegans Trigger
The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the trigger. In addition, sequencing analysis results shown below also confirms the presence of the trigger in comparison to the trigger sequences in the reference paper (Green et al. 2014). The biobricked trigger's (BBa_K2974400) function has been validated through experimentation and sequencing.


Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.

Sequencing Analysis


Figure 2: Lambert iGEM aligned the Trigger insert with the Eurofins Genomics sequencing results. This showed that the Trigger was successfully cloned.

===Applications of BBa_K2974400=== ===User Reviews=== BBa_K2974400 StartReviews BBa_K2974400 EndReviews