Difference between revisions of "Part:BBa K2967011"

 
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<partinfo>BBa_K2967011 short</partinfo>
 
<partinfo>BBa_K2967011 short</partinfo>
  
It is the sequence of inducible promoter P''hrpL''. HrpR([https://parts.igem.org/Part:BBa_K2967008, BBa_K2967008]) protein binds to HrpS([https://parts.igem.org/Part:BBa_K2967009, BBa_K2967009]) protein forming a complex and then triggering the transcription of induced promoter P''hrpL''. It functions like a AND logic gate to open an gene circuit.
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It is the sequence of inducible promoter P''hrpL''. HrpR([https://parts.igem.org/Part:BBa_K2967008 BBa_K2967008]) protein binds to HrpS([https://parts.igem.org/Part:BBa_K2967009 BBa_K2967009]) protein forming a complex and then triggering the transcription of induced promoter P''hrpL''. It functions like a AND logic gate to open an gene circuit.
 
The hrpL promoter (P''hrpL'') contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.  
 
The hrpL promoter (P''hrpL'') contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.  
  

Latest revision as of 11:04, 21 October 2019


The inducible promoter, PhrpL

It is the sequence of inducible promoter PhrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter PhrpL. It functions like a AND logic gate to open an gene circuit. The hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]