Difference between revisions of "Part:BBa K3110004"
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<partinfo>BBa_K3110004 short</partinfo> | <partinfo>BBa_K3110004 short</partinfo> | ||
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<partinfo>BBa_K3110004 parameters</partinfo> | <partinfo>BBa_K3110004 parameters</partinfo> | ||
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+ | <h1>Usage and Biology</h1> | ||
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+ | L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a strong RBS. | ||
+ | lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower concentrations of lldD by varying the strength of the Promoter and RBS controlling its production. | ||
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+ | <h1>Characterization</h1> | ||
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+ | Due to shortage of time we couldn't characterize it. |
Latest revision as of 10:56, 21 October 2019
Strong Promoter Strong RBS lldD Terminator
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1202
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 639
Usage and Biology
L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower concentrations of lldD by varying the strength of the Promoter and RBS controlling its production.
Characterization
Due to shortage of time we couldn't characterize it.