Difference between revisions of "Part:BBa K2974400:Experience"
| Line 13: | Line 13: | ||
</html> | </html> | ||
| − | <I>Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.</I> | + | <center><I>Figure 1: This graph compares the mean lux values of the C. elegans toehold switch, trigger, and the dual plasmid. The toehold switch and trigger have very similar lux values to LB and plain cells, whereas the dual plasmid has a higher lux value, indicating fluorescence.</I></center> |
<strong>Sequencing Analysis</strong> | <strong>Sequencing Analysis</strong> | ||
| Line 20: | Line 20: | ||
<center><img src="https://2019.igem.org/wiki/images/1/1a/T--Lambert_GA--CETriggerSequenceMegan.png" height="400px"> | <center><img src="https://2019.igem.org/wiki/images/1/1a/T--Lambert_GA--CETriggerSequenceMegan.png" height="400px"> | ||
</center> | </center> | ||
| + | <i><center>Figure 2: Lambert iGEM aligned the Trigger insert with the Eurofins Genomics sequencing results. This showed that the Trigger was successfully cloned.</center></i> | ||
| + | |||
</body> | </body> | ||
</hmtl> | </hmtl> | ||
Revision as of 10:54, 21 October 2019
T7 C. elegans Trigger
The results for the trigger were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the trigger. In addition, sequencing analysis results shown below also confirms the presence of the trigger in comparison to the trigger sequences in the reference paper (Green et al. 2014). The biobricked trigger's (BBa_K2974400) function has been validated through experimentation and sequencing.
Sequencing Analysis
