Difference between revisions of "Part:BBa K108014:Design"
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===Source=== | ===Source=== | ||
| − | Alcaligenes eutrophus H16 genome | + | PR was cloned from Alcaligenes eutrophus H16 genome (reference 1 and 2). |
===References=== | ===References=== | ||
Revision as of 06:06, 25 October 2008
PR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 136
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 using site-directed mutation.
Source
PR was cloned from Alcaligenes eutrophus H16 genome (reference 1 and 2).