Difference between revisions of "Part:BBa K108009:Design"

 
(References)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K108009 short</partinfo>
 
<partinfo>BBa_K108009 short</partinfo>
Line 13: Line 12:
 
===Source===
 
===Source===
  
E.coli K12 DH10B genome
+
FliK was cloned from E.coli K12 DH10B genome (reference 1).
  
 
===References===
 
===References===
 +
 +
1. NCBI.

Latest revision as of 05:28, 25 October 2008

FliK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 483


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

FliK was cloned from E.coli K12 DH10B genome (reference 1).

References

1. NCBI.