Difference between revisions of "Part:BBa K3117004"

Revision as of 10:35, 21 October 2019

((Glycine)x4-Serine)x4 Linker

The linker BBa_K3117004 consists of four repeats of four times Glycin and one time Serine. (Gly4Ser)4 linker is one of the most widely used flexible linker that can sustain the bioactivity of fusion proteins. It was used by 2019 FAU_Erlangen to create a bispecific antibody targeting GPA33 on colon cancer cells and CD3 on T cells (BBa_K3117029).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3117004 short</partinfo>
-The linker BBa_K3117004 consists of four repeats of four times Glycin and one time Serine. (Gly4Ser)4 linker is one of the most widely used flexible linker that can sustain the bioactivity of fusion proteins. It was used by 2019 FAU_Erlangen to create a bispecific antibody targeting GPA33 on colon cancer cells and CD3 on T cells.
+The linker BBa_K3117004 consists of four repeats of four times Glycin and one time Serine. (Gly4Ser)4 linker is one of the most widely used flexible linker that can sustain the bioactivity of fusion proteins. It was used by 2019 FAU_Erlangen to create a bispecific antibody targeting GPA33 on colon cancer cells and CD3 on T cells (BBa_K3117029).
@@ Line 9: / Line 9: @@
 ===Usage and Biology===
+Our flexible linker is composed of small, non-polar (e.g. Gly) and polar (e.g. Ser or Thr) amino acids. By adjusting the copy number, in this case 4 repeats of GGGGS, the length of this GS linker can be optimized to achieve appropriate separation of the proteins of interest [1]. 4 repeats ensure a high level of flexibility.
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 <span class='h3bb'>Sequence and Features</span>