Difference between revisions of "Part:BBa K3110050"
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<partinfo>BBa_K3110050 short</partinfo> | <partinfo>BBa_K3110050 short</partinfo> | ||
| − | + | The original YebF part can be found here: [[Part:BBa_K1659003]] | |
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<partinfo>BBa_K3110050 parameters</partinfo> | <partinfo>BBa_K3110050 parameters</partinfo> | ||
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| + | <h1>Usage and Biology</h1> | ||
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| + | YebF is a 13kDa protein that is known to be secreted into the extracellular medium by E. coli strains used in the lab. This particular protein was used with IL-12 in our design, in order to mediate the secretion of the cytokine from the bacteria. The part was used with both constitutive and inducible promoters, but the results were inconclusive. Western blots were done with both the cell pellet and concentrated supernatant but the protein was not detected. Possible errors could be because of SOEing errors in the promoter and RBS regions. | ||
Latest revision as of 10:34, 21 October 2019
YebF-IL12-FLAG
The original YebF part can be found here: Part:BBa_K1659003
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
YebF is a 13kDa protein that is known to be secreted into the extracellular medium by E. coli strains used in the lab. This particular protein was used with IL-12 in our design, in order to mediate the secretion of the cytokine from the bacteria. The part was used with both constitutive and inducible promoters, but the results were inconclusive. Western blots were done with both the cell pellet and concentrated supernatant but the protein was not detected. Possible errors could be because of SOEing errors in the promoter and RBS regions.