Difference between revisions of "Part:BBa K3081004"
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Here, we use the pTAC promoter to drive the expression of sfGFP and we can observe the intensity of fluorescense to obtain a proper inducing concentraion. | Here, we use the pTAC promoter to drive the expression of sfGFP and we can observe the intensity of fluorescense to obtain a proper inducing concentraion. | ||
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https://2019.igem.org/wiki/images/6/68/T--Peking--pTAC.png | https://2019.igem.org/wiki/images/6/68/T--Peking--pTAC.png | ||
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Figure 1: IPTG-induced GFP expression level tested by cytometer | Figure 1: IPTG-induced GFP expression level tested by cytometer | ||
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Latest revision as of 10:23, 21 October 2019
pTAC GFP
pTAC is a lactose-inducible promoter ,and it is also effectively induced by isopropyl-β-D-1-thiogalactoside (IPTG), which can diffuse across the cell membrane without the aid of the lactose transporter. This classic operon consists of two basic parts, a cassette for the constitutive expression of the repressor LacI and a cassette for the inducible expression of the target gene. The LacI can bind to the lac operator (lacO) region in the second cassette and trigger the repression. This repression stops transcription from the promoter itself. The derepression of transcription is carried out by inducers such as IPTG, which can remove LacI from the operator region by binding to it. Here, we use the pTAC promoter to drive the expression of sfGFP and we can observe the intensity of fluorescense to obtain a proper inducing concentraion.
Figure 1: IPTG-induced GFP expression level tested by cytometer
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1472