Difference between revisions of "Part:BBa K3061002"
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<h3>1. biology</h3> | <h3>1. biology</h3> | ||
<p>Channelrhodopsin 2(ChR2) is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc(BBa_K3061010链接)around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):<p> | <p>Channelrhodopsin 2(ChR2) is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc(BBa_K3061010链接)around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):<p> | ||
− | + | <img src="https://static.igem.org/mediawiki/parts/3/3d/T--DUT_China_B--BBa_K3061002.png"> | |
+ | <p>Figure 1. Results of SignalP 4.1 server</p> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 10:19, 21 October 2019
Sp, signal peptide for Chlamydomonas reinhardtti
It is an N-terminal signal peptide and destined the protein to be either secreted, extracellular or periplasmic. They can be used for exogenous expression of proteins.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 52
Illegal NgoMIV site found at 61 - 1000COMPATIBLE WITH RFC[1000]
1. biology
Channelrhodopsin 2(ChR2) is light-gated ion channels acting as sensory photoreceptors in Chlamydomonas reinhardtii. It is located in the plasma membrane. ChR2 depolarize the plasma membrane in the eyespot region on a millisecond time scale after light excitation. We use the signal peptide of ChR2 to locate fusion protein phyB_C-rluc(BBa_K3061010链接)around eyespot. The signal peptide correctly predicted by SignalP 4.1 server (predicts signal peptides) (Figure 1):
Figure 1. Results of SignalP 4.1 server