Difference between revisions of "Part:BBa K3061001"
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<h3>2.Characterization</h3><br><br> | <h3>2.Characterization</h3><br><br> | ||
We characterized the effect of expression through measuring movement features of VCHR-engineered alga. Measuring protocols see Characeriztion of the motility features of Chlamydomonas Reinhardtii.(黄色部分设置连接到protocol相应pdf)<br> | We characterized the effect of expression through measuring movement features of VCHR-engineered alga. Measuring protocols see Characeriztion of the motility features of Chlamydomonas Reinhardtii.(黄色部分设置连接到protocol相应pdf)<br> | ||
− | <h3>3. | + | <h3>3.Characterization</h3><br><br> |
− | Under standard protocols of alga movement measuring, the movement data of | + | <h2>3.1 Protocols</h2> |
− | + | We characterized the effect of expression through measuring movement features of VchR-engineered alga. Measuring protocols see Characeriztion of the motility features of Chlamydomonas Reinhardtii.(黄色部分设置连接到protocol相应pdf) | |
− | + | <h2>3.2 Result and discussion</h2> | |
− | + | Under standard protocols of alga movement measuring, the movement data of VchR-engineered Chlamydonomas is showed in figure 1. As we can see from the figure, VchR-engineered Chlamydonomas is able to move under light of 590nm.The moving speed of alga declines with light intensity, until showing random movement pattern at 32.6lx with almost none phototaxis pattern. VchR-engineered C. Reinhardtii can move at 0.1783±0.0072 mm/s under the blue light of 25.40lx. We can learn that VchR responses in light of 590nm more weakly than 480nm. | |
+ | <img src="https://static.igem.org/mediawiki/parts/b/bd/T--DUT_China_B--VCHR.pNG"> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 09:35, 21 October 2019
VchR, Channel rhodopsins of Volvox carteri
It is a kind of photochromic proteins that are activated under wavelength of 589nm. Photocurrent can be produced after activation. The codon usage was optimized to Chlamydomonas reinhardtti.
Sequence and Fe1111111111111111111111111111atures
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 994
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 400
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1400
Illegal SapI site found at 2307
Illegal SapI.rc site found at 261
1.Introduction
Channelrhodopsins are light-gated ion channels involved in the photoresponses of microalgae. We know that VChRs serve as primary phototaxis photoreceptors in Volvox because the photocurrents of VChR in vitro are similar to those recorded in Volvox cells and because the proteins are closely related to their counterparts in Chlamydomonas[1]. Since VCHR has a high similarity with CHR2 and its absorption spectrum range is 100 nm (about 580 nm) reddened by CHR2, we expressed the channel rhodopsin VCHR in C. reinhardtii. In order to broaden the sensitometric spectrum of Chlamydomonas reinhardtii.
2.Characterization
We characterized the effect of expression through measuring movement features of VCHR-engineered alga. Measuring protocols see Characeriztion of the motility features of Chlamydomonas Reinhardtii.(黄色部分设置连接到protocol相应pdf)