Difference between revisions of "Part:BBa K2970001"
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Trigger 2 forms together with Trigger 1 (<partinfo>BBa_K2970000</partinfo>) a complex due to complementary regions. This complex can open a gate (<partinfo>BBa_K2970002</partinfo>) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can occur. The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
 
Trigger 2 forms together with Trigger 1 (<partinfo>BBa_K2970000</partinfo>) a complex due to complementary regions. This complex can open a gate (<partinfo>BBa_K2970002</partinfo>) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can occur. The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.
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<img width="100%" src="https://2019.igem.org/wiki/images/b/bc/T--Hamburg--PartsFigure11.jpeg">
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<b>Figure 1: </b>A) Formation of trigger complex after translation. B) mRNA of gate sequence forms secondary structures that hide the ribosome binding site and the start codon.
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<img width="90%" src="https://2019.igem.org/wiki/images/4/49/T--Hamburg--Part_Figure6.jpg">
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<b>Figure 2: </b>Opening of the gate due to annealing of trigger complex to gate.
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To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (<partinfo>BBa_K2970004</partinfo>).  
 
To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (<partinfo>BBa_K2970004</partinfo>).  
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After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate will be opened, and the translation of the gene of interest can take place.
 
After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate will be opened, and the translation of the gene of interest can take place.
  

Revision as of 09:10, 21 October 2019


Trigger 2

Trigger 2 forms together with Trigger 1 (BBa_K2970000) a complex due to complementary regions. This complex can open a gate (BBa_K2970002) to enable translation of a gene that is locked with this gate to regulate translation. After binding of the trigger complex due to complementarity, the hairpin of the gate will open and release the ribosome binding site and the start codon, thus translation can occur. The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the loop). A single trigger cannot open the gate because it has only half the required complementary sequence.

Figure 1: A) Formation of trigger complex after translation. B) mRNA of gate sequence forms secondary structures that hide the ribosome binding site and the start codon.
Figure 2: Opening of the gate due to annealing of trigger complex to gate.
To transform the trigger into bacteria it should be put on a plasmid together with a promoter and a terminator as we did (BBa_K2970004). After transformation of both trigger plasmids and the gate plasmid in one bacterium all three mRNA structures will be formed, the gate will be opened, and the translation of the gene of interest can take place.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]