Difference between revisions of "Part:BBa K3286207"

Latest revision as of 08:54, 21 October 2019

RpfCch (TMD) sfGFP fusion

This part is similar to part BBa_K3286206, but in this strategy, only the transmembrane domain (TMD) was used to fuse with sfGFP. So again, this part was constructed to test whether part BBa_K3286200 was getting transcribed, translated and transported to the membrane.

Figure 1: Transformation of this part in E. coli Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.

Figure 2: Transformation of this part in E. coli Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.

Figure 3: Transformation of an E. coli Dh5-Alpha strain producing GFP, incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.

Figure 1 and 2 are showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges, especially compared to figure 3 which serves as a positive control. Indicating that this part (but also BBa_K3286200) is getting transcribed, translated and transported to the membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 227
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 661
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This part is similar to part <partinfo>BBa_K3286206</partinfo>, but in this strategy, only the transmembrane domain (TMD) was used to fuse with sfGFP. So again, this part was constructed to test whether part <partinfo>BBa_K3286200</partinfo> was getting transcribed, translated and transported to the membrane.
-[[File:T--Wageningen UR--rpfC-GFP 3 IJ H.jpg|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
+[[File:T--Wageningen UR--rpfC-GFP 3 IJ H.jpg|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]][[File:T--Wageningen UR--rpfC-GFP 3 IJ I.jpg|thumb|center|<b>Figure 2:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
-[[File:T--Wageningen UR--rpfC-GFP 3 IJ I.jpg|750px|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
+[[File:T--Wageningen UR--GFP Pos 2 IJ D.jpg|thumb|center|<b>Figure 3:</b> Transformation of an <i>E. coli</i> Dh5-Alpha strain producing GFP, incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
-Figure 1 and 2 are showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges. Indicating that this part (but also <partinfo>K3286200</partinfo>) is getting transcribed, translated and transported to the membrane.
+Figure 1 and 2 are showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges, especially compared to figure 3 which serves as a positive control. Indicating that this part (but also <partinfo>K3286200</partinfo>) is getting transcribed, translated and transported to the membrane.