Difference between revisions of "Part:BBa K3286206"

Latest revision as of 08:47, 21 October 2019

RpfCch sfGFP fusion

This part is a fusion of two previously presented parts BBa_K3286200, BBa_K3286203. This part was made to test whether part BBa_K3286200 was getting transcribed, translated and transported to the membrane.

Figure 1: Transformation of this part in E. coli Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.

Figure 2: Transformation of an E. coli Dh5-Alpha strain producing GFP, incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.

Figure 1 is showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges, especially compared to figure 2 which serves as a positive control. Indicating that this part (but also BBa_K3286200) is getting transcribed, translated and transported to the membrane.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 227
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 757
Illegal NgoMIV site found at 848
Illegal NgoMIV site found at 1675
Illegal NgoMIV site found at 1770
Illegal AgeI site found at 2326
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 [[File:T--Wageningen UR--rpfC-GFP 3 IJ G.jpg|750px|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
-[[File:File:T--Wageningen UR--GFP Pos 2 IJ D.jpg|750px|thumb|center|<b>Figure 2:</b> Transformation of an <i>E. coli</i> Dh5-Alpha strain producing GFP, incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
+[[File:T--Wageningen UR--GFP Pos 2 IJ D.jpg|750px|thumb|center|<b>Figure 2:</b> Transformation of an <i>E. coli</i> Dh5-Alpha strain producing GFP, incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
 Figure 1 is showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges, especially compared to figure 2 which serves as a positive control. Indicating that this part (but also <partinfo>K3286200</partinfo>) is getting transcribed, translated and transported to the membrane.