Difference between revisions of "Part:BBa K3286206"
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
[[File:T--Wageningen UR--rpfC-GFP 3 IJ G.jpg|750px|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
 
[[File:T--Wageningen UR--rpfC-GFP 3 IJ G.jpg|750px|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
 +
[[File:T--Wageningen UR--GFP Pos 2 IJ D.jpg|750px|thumb|center|<b>Figure 2:</b> Transformation of an <i>E. coli</i> Dh5-Alpha strain producing GFP, incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]]
  
−
Figure 1 is showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges. Indicating that this part (but also <partinfo>K3286200</partinfo>) is getting transcribed, translated and transported to the membrane.
+
Figure 1 is showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges, especially compared to figure 2 which serves as a positive control. Indicating that this part (but also <partinfo>K3286200</partinfo>) is getting transcribed, translated and transported to the membrane.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:47, 21 October 2019


RpfCch sfGFP fusion

This part is a fusion of two previously presented parts BBa_K3286200, BBa_K3286203. This part was made to test whether part BBa_K3286200 was getting transcribed, translated and transported to the membrane.

Figure 1: Transformation of this part in E. coli Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.
Figure 2: Transformation of an E. coli Dh5-Alpha strain producing GFP, incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.

Figure 1 is showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges, especially compared to figure 2 which serves as a positive control. Indicating that this part (but also BBa_K3286200) is getting transcribed, translated and transported to the membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 848
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 1770
    Illegal AgeI site found at 2326
  • 1000
    COMPATIBLE WITH RFC[1000]