Difference between revisions of "Part:BBa K3137011"
Line 7: Line 7:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to (([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006])([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005])) display green fluorescence.
+
When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to ([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005])display green fluorescence.
  
  

Revision as of 08:39, 21 October 2019


gfp

The gfp can express green fluorescent protein, which can be used as a marker gene in the process to report the number of cells.

Usage and Biology

When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to (BBa_K3137005)display green fluorescence.


Characterization of PputA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644