Difference between revisions of "Part:BBa K3268001"

 
 
Line 6: Line 6:
  
 
In order to display mOR103-15 on the surface of cell, we found that the Lpp′OmpA(46-159) hybrid protein can serve as an efficient targeting vehicle for localizing a variety soluble proteins onto the E. coli surface.We got the part BBa_J36850 which is combined with a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-159, and streptavidin wild-type + His6 tag from iGEM kit 2019.
 
In order to display mOR103-15 on the surface of cell, we found that the Lpp′OmpA(46-159) hybrid protein can serve as an efficient targeting vehicle for localizing a variety soluble proteins onto the E. coli surface.We got the part BBa_J36850 which is combined with a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-159, and streptavidin wild-type + His6 tag from iGEM kit 2019.
−
 
+
<br>
−
 
+
<br>
 +
<html>
 +
<div>
 +
<img src="https://2019.igem.org/wiki/images/1/15/T--NYMU-Taipei--cloning_figure_6.jpg" width="60%" height="60%">
 +
</div>
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:38, 21 October 2019


Lac-inducible generator of Lpp-OmpA(46-159)-mOR103-15 + His6 tag

This part contains a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-159, and mOR103-15 + His6 tag. This expression should display streptavidin on the cell surface of E. coli.

In order to display mOR103-15 on the surface of cell, we found that the Lpp′OmpA(46-159) hybrid protein can serve as an efficient targeting vehicle for localizing a variety soluble proteins onto the E. coli surface.We got the part BBa_J36850 which is combined with a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-159, and streptavidin wild-type + His6 tag from iGEM kit 2019.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1463