Difference between revisions of "Part:BBa K3113069"
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We generated helical bundle heterodimers in which each monomer is a helix–turn–helix starting from four-helix backbones produced using a generalization of the Crick coiled-coil parameterization.<ref>Chen, Zibo ; Boyken, Scott E; Jia, Mengxuan ; Busch, Florian ; Flores-Solis, David ; Bick, Matthew J; Lu, Peilong ; VanAernum, Zachary L; Sahasrabuddhe, Aniruddha ; Langan, Robert A; Bermeo, Sherry ; Brunette, T J; Mulligan, Vikram Khipple ; Carter, Lauren P; DiMaio, Frank ; Sgourakis, Nikolaos G; Wysocki, Vicki H; Baker, David, Programmable design of orthogonal protein heterodimers Nature, 2018, ISSN: 1476-4687.</ref>
 
We generated helical bundle heterodimers in which each monomer is a helix–turn–helix starting from four-helix backbones produced using a generalization of the Crick coiled-coil parameterization.<ref>Chen, Zibo ; Boyken, Scott E; Jia, Mengxuan ; Busch, Florian ; Flores-Solis, David ; Bick, Matthew J; Lu, Peilong ; VanAernum, Zachary L; Sahasrabuddhe, Aniruddha ; Langan, Robert A; Bermeo, Sherry ; Brunette, T J; Mulligan, Vikram Khipple ; Carter, Lauren P; DiMaio, Frank ; Sgourakis, Nikolaos G; Wysocki, Vicki H; Baker, David, Programmable design of orthogonal protein heterodimers Nature, 2018, ISSN: 1476-4687.</ref>
  
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<h2>Biology</h2>
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<h2>Characterization</h2>
  
  

Revision as of 08:37, 21 October 2019

DHD154b

DHD154b is a double coiled-coil structure.

Usage

To increase the modularity of our system we decided to test coiled coil proteins. These coiled coils mimic the interactions of DNA helix-helix interactions. The coiled-coil protein interaction allows the loading of more than one protein and larger proteins. We are using these helical structures to load RNA binding proteins into exosomes or virus-like-particles.

Biology

We generated helical bundle heterodimers in which each monomer is a helix–turn–helix starting from four-helix backbones produced using a generalization of the Crick coiled-coil parameterization.[1]

Characterization


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

  1. ↑ Chen, Zibo ; Boyken, Scott E; Jia, Mengxuan ; Busch, Florian ; Flores-Solis, David ; Bick, Matthew J; Lu, Peilong ; VanAernum, Zachary L; Sahasrabuddhe, Aniruddha ; Langan, Robert A; Bermeo, Sherry ; Brunette, T J; Mulligan, Vikram Khipple ; Carter, Lauren P; DiMaio, Frank ; Sgourakis, Nikolaos G; Wysocki, Vicki H; Baker, David, Programmable design of orthogonal protein heterodimers Nature, 2018, ISSN: 1476-4687.