Difference between revisions of "Part:BBa K3113069"
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DHD154b is a double coiled-coil structure. | DHD154b is a double coiled-coil structure. | ||
| − | < | + | <h2>Usage</h2> |
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| + | To increase the modularity of our system we decided to test coiled coil proteins. These coiled coils mimic the interactions of DNA helix-helix interactions. The coiled-coil protein interaction allows the loading of more than one protein and larger proteins. We are using these helical structures to load RNA binding proteins into exosomes or virus-like-particles. | ||
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| + | <h2>Biology</h2> | ||
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| + | We generated helical bundle heterodimers in which each monomer is a helix–turn–helix starting from four-helix backbones produced using a generalization of the Crick coiled-coil parameterization.<ref>Chen, Zibo ; Boyken, Scott E; Jia, Mengxuan ; Busch, Florian ; Flores-Solis, David ; Bick, Matthew J; Lu, Peilong ; VanAernum, Zachary L; Sahasrabuddhe, Aniruddha ; Langan, Robert A; Bermeo, Sherry ; Brunette, T J; Mulligan, Vikram Khipple ; Carter, Lauren P; DiMaio, Frank ; Sgourakis, Nikolaos G; Wysocki, Vicki H; Baker, David, Programmable design of orthogonal protein heterodimers Nature, 2018, ISSN: 1476-4687.</ref> | ||
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| + | <h2>Biology</h2> | ||
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<partinfo>BBa_K3113069 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3113069 SequenceAndFeatures</partinfo> | ||
| + | <h2>Characterization</h2> | ||
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Revision as of 08:37, 21 October 2019
DHD154b
DHD154b is a double coiled-coil structure.
Usage
To increase the modularity of our system we decided to test coiled coil proteins. These coiled coils mimic the interactions of DNA helix-helix interactions. The coiled-coil protein interaction allows the loading of more than one protein and larger proteins. We are using these helical structures to load RNA binding proteins into exosomes or virus-like-particles.
Biology
We generated helical bundle heterodimers in which each monomer is a helix–turn–helix starting from four-helix backbones produced using a generalization of the Crick coiled-coil parameterization.[1]
Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
- ↑ Chen, Zibo ; Boyken, Scott E; Jia, Mengxuan ; Busch, Florian ; Flores-Solis, David ; Bick, Matthew J; Lu, Peilong ; VanAernum, Zachary L; Sahasrabuddhe, Aniruddha ; Langan, Robert A; Bermeo, Sherry ; Brunette, T J; Mulligan, Vikram Khipple ; Carter, Lauren P; DiMaio, Frank ; Sgourakis, Nikolaos G; Wysocki, Vicki H; Baker, David, Programmable design of orthogonal protein heterodimers Nature, 2018, ISSN: 1476-4687.