Difference between revisions of "Part:BBa K3286206"
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[[File:T--Wageningen UR--rpfC-GFP 3 IJ G.jpg|750px|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]] | [[File:T--Wageningen UR--rpfC-GFP 3 IJ G.jpg|750px|thumb|center|<b>Figure 1:</b> Transformation of this part in <i>E. coli</i> Dh5-Alpha cells incubated on a LB agar plate. Fluorescence is visualized by a fluorescence microscope.]] | ||
| − | Figure is showing | + | Figure 1 is showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges. Indicating that this part (but also <partinfo>K3286200</partinfo>) is getting transcribed, translated and transported to the membrane. |
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Revision as of 08:36, 21 October 2019
RpfCch sfGFP fusion
This part is a fusion of two previously presented parts BBa_K3286200, BBa_K3286203. This part was made to test whether part BBa_K3286200 was getting transcribed, translated and transported to the membrane.
Figure 1 is showing the observed fluorescence by this part. What can be observed is that cells are actually showing a higher intensity of fluorescence at the edges. Indicating that this part (but also BBa_K3286200) is getting transcribed, translated and transported to the membrane.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 227
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 757
Illegal NgoMIV site found at 848
Illegal NgoMIV site found at 1675
Illegal NgoMIV site found at 1770
Illegal AgeI site found at 2326 - 1000COMPATIBLE WITH RFC[1000]