Difference between revisions of "Part:BBa K3152005"
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3152005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3152005 SequenceAndFeatures</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
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We contructed it successfully and took out two red colonies from the LB solid medium and digested by EcoRI and PstI restriction enzymes to further verify the correctness of the connection.
 
We contructed it successfully and took out two red colonies from the LB solid medium and digested by EcoRI and PstI restriction enzymes to further verify the correctness of the connection.
  
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[[File:Antibiotics-R1.png|200px|thumb|left|Figure 1 Photos of transformants of “pSB1C3-mec operator-mCherry” on LB agar broth.]]
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[[File:Antibiotics-R1.png|300px|thumb|center|Figure 1 Photos of transformants of “pSB1C3-mec operator-mCherry” on LB agar broth.]]
  
  

Latest revision as of 08:28, 21 October 2019


mec operator-mCherry

This composite part is a report pathway. The mec operator is responsible for mCherry (BBa_J06504) expressing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

We contructed it successfully and took out two red colonies from the LB solid medium and digested by EcoRI and PstI restriction enzymes to further verify the correctness of the connection.

Figure 1 Photos of transformants of “pSB1C3-mec operator-mCherry” on LB agar broth.