Difference between revisions of "Part:BBa K3311002"
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<partinfo>BBa_K3311002 short</partinfo> | <partinfo>BBa_K3311002 short</partinfo> | ||
− | + | This composite part is a unit of “ HucR-pHucO-mCherry”(BBa_K3311004), which is responsible for mCherry (BBa_J06504) expressing. | |
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<partinfo>BBa_K3311002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3311002 SequenceAndFeatures</partinfo> | ||
+ | ===Usage and Biology=== | ||
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+ | The plasmid “pHucO-mCherry” was transformed to E. Coli DH5α for function test. As shown in Figure 1, The bacteria turned red after incubating overnight at 37 °C, which means the pHucO works well. | ||
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+ | [[File:PHucO.png|600px|thumb|center|Figure 1 Incubation of E. Coli containing the pSB1C3-pHucO-mCherry]] | ||
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Latest revision as of 08:07, 21 October 2019
pHucO-mCherry
This composite part is a unit of “ HucR-pHucO-mCherry”(BBa_K3311004), which is responsible for mCherry (BBa_J06504) expressing.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The plasmid “pHucO-mCherry” was transformed to E. Coli DH5α for function test. As shown in Figure 1, The bacteria turned red after incubating overnight at 37 °C, which means the pHucO works well.