Difference between revisions of "Part:BBa K3113007"
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| + | The informational readout of ALiVE is RNA. To load a specific RNA into the vesicles we fused it to RNA-motifs which are bound specifically by RNA binding proteins. Two combinations of binding protein and motif were tested. One was the archaeal RNA binding protein L7Ae which binds to the k-turns in the box C/D snoRNP. | ||
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| + | <h2>Biology</h2> | ||
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| + | The members of the L7Ae/15.5kD protein family that characteristically recognize K-turn motifs found in both archaeal and eukaryotic RNAs. In Archaea, the L7Ae protein uniquely binds the K-loop motif found in box C/D.<ref>Gagnon, Keith T et al. “Signature amino acids enable the archaeal L7Ae box C/D RNP core protein to recognize and bind the K-loop RNA motif.” RNA (New York, N.Y.) vol. 16,1 (2010): 79-90. doi:10.1261/rna.1692310</ref> | ||
| + | The k-turn is a ubiquitous structural motif in RNA forming a very tight kink in the axis of helical RNA that plays an important role in many aspects of RNA function. L7Ae is a member of a superfamily of proteins that bind k-turns in RNA, stabilizing the tightly kinked conformation. They are extremely widespread and are important in the assembly of RNA–protein complexes central to translation, splicing and site-specific RNA modification. <ref>Lilley, D. M. J. (2019). "The L7Ae proteins mediate a widespread and highly functional protein–RNA interaction." The Biochemist 41(2): 40-44.</ref> | ||
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| + | <h2>Characterization</h2> | ||
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<partinfo>BBa_K3113007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3113007 SequenceAndFeatures</partinfo> | ||
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Revision as of 07:41, 21 October 2019
C/D-Box
C/D box is a stemloop structure shown to associate with four evolutionary conserved and essential proteins including the archaeal homolog (L7Ae) of Snu13.
Usage
The informational readout of ALiVE is RNA. To load a specific RNA into the vesicles we fused it to RNA-motifs which are bound specifically by RNA binding proteins. Two combinations of binding protein and motif were tested. One was the archaeal RNA binding protein L7Ae which binds to the k-turns in the box C/D snoRNP.
Biology
The members of the L7Ae/15.5kD protein family that characteristically recognize K-turn motifs found in both archaeal and eukaryotic RNAs. In Archaea, the L7Ae protein uniquely binds the K-loop motif found in box C/D.[1] The k-turn is a ubiquitous structural motif in RNA forming a very tight kink in the axis of helical RNA that plays an important role in many aspects of RNA function. L7Ae is a member of a superfamily of proteins that bind k-turns in RNA, stabilizing the tightly kinked conformation. They are extremely widespread and are important in the assembly of RNA–protein complexes central to translation, splicing and site-specific RNA modification. [2]
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Gagnon, Keith T et al. “Signature amino acids enable the archaeal L7Ae box C/D RNP core protein to recognize and bind the K-loop RNA motif.” RNA (New York, N.Y.) vol. 16,1 (2010): 79-90. doi:10.1261/rna.1692310
- ↑ Lilley, D. M. J. (2019). "The L7Ae proteins mediate a widespread and highly functional protein–RNA interaction." The Biochemist 41(2): 40-44.