Difference between revisions of "Part:BBa K103014"
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<!-- Add more about the biology of this part here --> | <!-- Add more about the biology of this part here --> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | *It's fusion of E. coli outer membrane protein (OmpA) domain (BBa_K103006) with beta-lactamase subunit alpha (BBa_K103008) | + | *It's fusion of E. coli outer membrane protein (OmpA) domain ([[Part:BBa_K103006 | BBa_K103006]]) with beta-lactamase subunit alpha ([[Part:BBa_K103008 | BBa_K103008]]) |
*Fusion partners are connected via Gly-Ser-Gly linker | *Fusion partners are connected via Gly-Ser-Gly linker | ||
| − | * | + | *It's base for creation of hunter fusions in 'hunter and prey' selection system |
*Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane | *Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane | ||
| − | *Interaction between hunter and prey (fused with BBa_K103020) makes cells ampicillin resistant | + | *Interaction between hunter and prey (fused with [[Part:BBa_K103020 | BBa_K103020]]) makes cells ampicillin resistant |
*This part uses our NdeI/SacI/BamHI fusion cloning substandard | *This part uses our NdeI/SacI/BamHI fusion cloning substandard | ||
Revision as of 17:38, 24 October 2008
OmpA_linker_alpha_linker
Usage and Biology
- It's fusion of E. coli outer membrane protein (OmpA) domain ( BBa_K103006) with beta-lactamase subunit alpha ( BBa_K103008)
- Fusion partners are connected via Gly-Ser-Gly linker
- It's base for creation of hunter fusions in 'hunter and prey' selection system
- Hunter protein should be attached via the second linker (using BamHI+ Biobrick standard suffix enzyme). It will be exported outside the cell and attached to the outer membrane
- Interaction between hunter and prey (fused with BBa_K103020) makes cells ampicillin resistant
- This part uses our NdeI/SacI/BamHI fusion cloning substandard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1042
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]