Difference between revisions of "Part:BBa K3031015:Design"
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Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.  
 
Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.  
  
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<img src="T--SUIS_Shanghai--ironQSassay.png" alt="IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)" style="width:128px;height:128px;">
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<img src="https://2019.igem.org/wiki/images/9/9a/T--SUIS_Shanghai--ironQSassay.png" alt="IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)" style="width:128px;height:128px;">
  
  

Revision as of 06:14, 21 October 2019


LuxI with FUR box located in the -10 region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insertion of the FUR box was informed by the report by Chu et al., (2015). This study inserted the FUR box region at different locations on a plasmid containing the LuxR/LuxI QS system. The construct with the insert at the -10 region (termed ironQS2) performed the best in experiments testing different constructs (as seen below) in low iron environments.

<img src="T--SUIS_Shanghai--ironQSassay.png" alt="IronQS2 with the FUR box located at -10 position performed well at suppressing genes in low iron environments (in presence of iron chelator 2-2' Bipyridine)" style="width:128px;height:128px;">


Source

BioBrick sequences.

References

Chu, Teng, Ni, Chunshan, Zhang, Lingzhi, Wang, Qiyao, Xiao, Jingfan, Zhang, Yuanxing, & Liu, Qin. (2015). A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine. Microbial Cell Factories,14(1), 37.

Rai, N., Anand, R., Ramkumar, K., Sreenivasan, V., Dabholkar, S., Venkatesh, K., . . . Buchler, N. (2012). Prediction by Promoter Logic in Bacterial Quorum Sensing (Prediction by Promoter Logic in Quorum Sensing). PLoS Computational Biology,8(1), E1002361.