Difference between revisions of "Part:BBa K3145003:Design"
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− | + | Our original construct (pY71-bFMO) was cloned in our lab. After we performed site-directed mutagenesis to correct a point mutation in our T7 promoter and RBS region, our original construct was now identical to the J18912-bFMO construct. | |
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===References=== | ===References=== |
Revision as of 05:11, 21 October 2019
J18912-bFMO
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 240
Illegal AgeI site found at 263
Illegal AgeI site found at 567
Illegal AgeI site found at 897 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 20
Design Notes
There was an illegal XbaI cut site in between our T7 promoter and RBS region in our pY71-bFMO plasmid that was corrected through site-directed mutagenesis to make our construct identical to J18912-bFMO.
Source
Our original construct (pY71-bFMO) was cloned in our lab. After we performed site-directed mutagenesis to correct a point mutation in our T7 promoter and RBS region, our original construct was now identical to the J18912-bFMO construct.