Difference between revisions of "Part:BBa K3031019:Design"

 
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===Design Notes===
 
===Design Notes===
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Mutations of the OxyR protein.  
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The OxyR protein transcription factor was designed using principles relating to statistical data of structural features of reactive cysteines in a wide variety of proteins.
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Constitutive promoter was chosen for the production of the OxyR protein in order to reach a high enough level to be able to bind with H2O2 and induce the downstream genes controlled by TrxC promoter.  
  
  

Revision as of 04:55, 21 October 2019


Constitutive promoter + OxyR + TrxC promoter + GFP generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 781
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734
    Illegal BsaI.rc site found at 1894


Design Notes

The OxyR protein transcription factor was designed using principles relating to statistical data of structural features of reactive cysteines in a wide variety of proteins. Constitutive promoter was chosen for the production of the OxyR protein in order to reach a high enough level to be able to bind with H2O2 and induce the downstream genes controlled by TrxC promoter.


Source

Yang Ming University iGEM team 2013

OxyR sequence from Uniprot and then sequenced by Genscript.

References