Difference between revisions of "Part:BBa K3170709"
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<li>Cfp is a powerful tool for detecting nanoscale and nanoscale distance changes in biological macromolecules in vivo. It can be used to detect the direct interaction of two protein molecules in a cell. When FRET occurs in CFP/YFP, the energy of CFP is transferred to YFP when YFP is excited by 440nm light. When the YFP is bleached, the energy transfer process of CFP to YFP is interrupted. The weaker CFP became brighter, confirming FRET.</li> | <li>Cfp is a powerful tool for detecting nanoscale and nanoscale distance changes in biological macromolecules in vivo. It can be used to detect the direct interaction of two protein molecules in a cell. When FRET occurs in CFP/YFP, the energy of CFP is transferred to YFP when YFP is excited by 440nm light. When the YFP is bleached, the energy transfer process of CFP to YFP is interrupted. The weaker CFP became brighter, confirming FRET.</li> | ||
</ul> | </ul> | ||
| + | ==Experimental Results== | ||
[[File:T--LZU-CHINA--cfp.png|600px|thumb|center|]] | [[File:T--LZU-CHINA--cfp.png|600px|thumb|center|]] | ||
<ul> | <ul> | ||
Revision as of 04:14, 21 October 2019
CFP
cyan fluorescent protein
- Cfp is a powerful tool for detecting nanoscale and nanoscale distance changes in biological macromolecules in vivo. It can be used to detect the direct interaction of two protein molecules in a cell. When FRET occurs in CFP/YFP, the energy of CFP is transferred to YFP when YFP is excited by 440nm light. When the YFP is bleached, the energy transfer process of CFP to YFP is interrupted. The weaker CFP became brighter, confirming FRET.
Experimental Results
- Imaging begins approximately 2 hours after the media is replaced with a fluorescent imaging medium. Get images every 1 hour. SW1990 cells were observed using 440 nm excitation light, and the cells turned from cyan to yellow.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 694
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]